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KMID : 0848120070320020079
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International Journal of Oral Biology
2007 Volume.32 No. 2 p.79 ~ p.84
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Mutantional analysis of tumor suppressor gene p53 in human oral squamous carcinoma cell line YD-9
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Min Ji-Hak
Kim Do-Kyun
Lee Moo-Hyung
Bae Moon-Kyoung
Um Kyung-Il
Kwak Hyun-Ho
Park Bong-Soo
Kim Gyoo-Cheon
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Abstract
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Oral squamous carcinoma (OSC) is the most common malignant neoplasm of the oral mucosa. Although the etiology of OSC is not fully understood, accumulated evidences indicate that the activation of proto-oncogenes and the inactivation of tumor suppressor genes underlie the disease development. An OSC cell line, YD-9 was newly established and characterized. However, the mutational analysis of p53 gene was not performed. Thus, in this study, the presence of mutation in the p53 gene was examined by amplification of exon-4 to -8 and subsequent DNA sequencing. Two point mutations were found in exon-4 and -6: A to G, resulting in amino acid change Tyr to Cys in exon-4, and C to G, resulting in amino acid change Gly to Arg in exon-6, respectively. Any mutation was not found in the exon-5, -7 and -8. The presented results would contribute to basic research to understand the biological mechanism of OSC using YD-9 cells.
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KEYWORD
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mutation of p53, oral squamous carcinoma
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